Related Papers
XXII Congress of the International Society on Thrombosis and Haemostasis. Kyoto, Japan. 23 – 28/07/2011
XXII Congress of the International Society on Thrombosis and Haemostasis. Kyoto, Japan. 23 – 28/07/2011
2011 •
Darío Fernández
The restricted deletion of the podocalyxin gene in megakaryocytes perturbs the control of hemostasia. Podocalyxin (Podxl) is an adhesive sialoprotein of the CD34 family, expressed in platelets and exposed at the membrane surface upon stimulation by physiological agonists, suggesting a regulatory role in the platelet function(s). This work aimed at investigating the role of Podxl in platelet function and hemostasia. To elucidate the role of Podxl in platelets we used the Cre-LoxP gene targeting methodology to generate mice with a restricted ablation of the podxl gene in megakaryocytes. Mice with Podxl-null megakaryocytes did not produce any apparent phenotypical changes, showing a normal life span and fertility, and normal blood cells count. However, the bleeding time was retarded and the aggregation of platelets in response to physiological agonists was diminishsed in Podxl-null platelets. The number, size-distribution and polyploidy were similar in nul-Podxl megakaryocytes to the floxed controls. Podxl-null platelets exhibited normal surface receptors content and normal stimulation by agonists. However, the ferric chloride-induced occlusion of the carotid artery was significantly retarded in the platelet Podxl-null mice. Moreover, perfusion of uncoagulated blood from Podxl-null mice in parallel flow chamber showed reduced platelet adhesion and formation of aggregates. To conclude, megakaryocyte/platelets Podxl is involved in the regulation of hemostasis, likely acting as a platelet co-stimulator due to its proadhesive properties.
International Journal of Obesity
Polymorphisms of matrix metalloproteinase gene and adiposity indices in European children: results of the IDEFICS study
2013 •
T. Veidebaum
Research and Practice in Thrombosis and Haemostasis
Illustrated State‐of‐the‐Art Capsules of the ISTH 2019 Congress in Melbourne, Australia
2019 •
Jeffrey Weitz
Transplantation Proceedings
Management of Kidney Transplantation in a Factor VII-Deficient Patient: Case Report
2012 •
Vincenzo Rizza
Journal of Thrombosis and Haemostasis
Wednesday, 27 July 2011
2011 •
Marianne Severinsen
Thrombosis and Haemostasis
Added value of blood cells in thrombin generation testing
2021 •
Joke Konings
The capacity of blood to form thrombin is a critical determinant of coagulability. Plasma thrombin generation (TG), a test that probes the capacity of plasma to form thrombin, has improved our knowledge of the coagulation system and shows promising utility in coagulation management. Although plasma TG gives comprehensive insights in the function of pro- and anticoagulation drivers, it does not measure the role of blood cells in TG. In this literature review, we discuss currently available continuous TG tests that can reflect the involvement of blood cells in coagulation, in particular the fluorogenic assays that allow continuous measurement in platelet rich plasma and whole blood. We also provide an overview about the influence of blood cells on blood coagulation, with emphasis on the direct influence of blood cells on TG. Platelets accelerate the initiation and velocity of thrombin generation by phosphatidylserine exposure, granule content release and surface receptor interaction w...
Frequency and association between polimorphism MTHFR gene, folic acid vitamin B12 and mild hyperhom*ocysteinemia in pediatric stroke
Jasna Lenicek Krleza, Ana Bronić, Goran Ferenčak
Introduction: In pediatric stroke, the cause of brain infarction remains unexplaines in 20–40%. Hypercoagulability may account for a significant proportion of unexplained strokes. hom*ocystein (Hcy) and him many-sided effect on hypercoagulability are good established in adults and children with different illness. For beginning mild hyperhom*ocysteinemia mostly responsible are inadequate taken with low folic acid and vitamin B12. Mutation for methylenetetrahydrofolate reductase (MTHFR) gene, too. Aim: Establish frequency of decrease values for folic acid and vitamin B12, increase values for Hcy and incidence mutation for MTHFR gene. Answer to which degree disorder folic acid pathway have effect on remetilation Hcy and beginning mild hyperhom*ocysteinemia in children with ischemic stroke. Materials and methods: Frothy-five children (0-18 years) with ischemic stroke studied. Diagnosis of stroke was established after evidence of cerebral infarction on brain with computed tomography scan. H...
Research and practice in thrombosis and haemostasis
Advances in Clinical and Basic Science of Coagulation: Illustrated abstracts of the 9th Chapel Hill Symposium on Hemostasis
2018 •
Hartmut Weiler
This 9th Symposium on Hemostasis is an international scientific meeting held biannually in Chapel Hill, North Carolina. The meeting is in large measure the result of the close friendship between the late Dr. Harold R. Roberts of UNC Chapel Hill and Dr. Ulla Hedner of Novo Nordisk. When Novo Nordisk was developing the hemophilia therapy that would become NovoSeven, they sponsored a series of meetings to understand the basic biology and clinical applications of factor VIIa. The first meeting in Chapel Hill was held April 4-6, 2002 with Dr. Roberts as the organizer. Over the years, the conference emphasis has expanded from discussions of factor VIIa and tissue factor to additional topics in hemostasis and thrombosis. This year's meeting includes presentations by internationally renowned speakers that discuss the state-of-the-art on an array of important topics, including von Willebrand factor, engineering advances, coagulation and disease, tissue factor biology, therapeutic advance...
Tarek Hegazi
Pathophysiology of Haemostasis and Thrombosis
D-dimer to fibrinogen ratio in geriatric patients with hip injuries
2008 •
Ana Bronić
Recently it has been suggested that a combination of functional fibrinogen and D-dimer measurement (D/Fratio) above certain cut off is highly specific for the presence of thromboembolic events among patients with clinical symptoms of the disease. We investigated, retrospectively, D/Fratio preformance in patients with hip injuries referred to our hospital during one year. D-dimers and fibrinogen were measured in plasma samples by immunofiltration (Nycocard ReaderII, AxisShield, Norway) and Clauss method (Dade Behring ; Sysmex CA1500 analyzer), respectively. Data were reported as proportions and medians with range, and as appropriate were compared by the Mann– Whitney test. On admission, obtained D-dimer values, fibrinogen and D/Fratio in 40 consecutive patients were 2.6(0.6-5.5) mg/L, 3.5(2.9-4.6)g/L and 0.8(0.2-1.7), respectively. According to comprehensive physician report and doppler ultrasonography, two groups were considered: GI, group of patients(26/40) without and GII, group w...
